Faculty

 

 Madhu Khanna

 


 

CURRICULUM VITAE

Address

Work

Associate Professor
Department of Virology

VP Chest Institute

Delhi University, Delhi 110007

New Delhi -110012

India
Telephone:      +91-11-27402432

 

 

 

Home

DII/3, Flats

I.A.R.I. Campus, Pusa

New Delhi -110012

India

Telephone:      91 11 25841210

Mobile: 9958524922

Email: madhukhanna@hotmail.com

 

 

 

Personal Details

 

Marital status: Married
Nationality: Indian

Date of Birth 24.06.64

 

Education

Ph.D. (Lucknow University) from KG. Medical College, Department of Virology Thesis supervisor Professor UC Chaturvedi, Head, Department of Microbiology, KG Medical College Lucknow, India.  

 

Thesis title Purification and characterization of dengue virus induced cytotoxic factor.

 

 

 

 

 

Research Interests

 

·       Molecular diagnosis of influenza viruses

·       Pathogenesis of influenza virus

·       Antiviral

Professional Affiliations

·       Life member: Indian Immunology Society

·       Life member: Biotechnology Society of India

·       Life member influenza foundation of India

·       International member ICEID

·       International member ISIRV

·       Life member: Indian Virological Society

·       Secretary General: Biotechnology Society of India

·       Joint Secretary, International Association of Medical and Pharmaceutical Virologists

 

2. Teaching Experience

 

Name of the Institution

Designation with pay scale

Nature of post

Classes taught/type of duties

Period

FromTo

Total Experience

Vallabhbhai Patel Chest Institute

Associate Professor

 

Permanent

Research / Teaching

MD Microbiology & M.Sc. Virology

(At ACBR)

20.10.97          to 

till date

13 years & 11 months

AIIMS Department of BiochemistryNew Delhi.

Research Officer

Temporary

Research & Teaching

Undergraduates (B.Sc)

1st May 1996 to                     23 June 1997

1 year

& 1 month

AIIMS Department of Biochemistry

Research Associate

Temporary

Research &

Teaching

Undergraduates (B.Sc.)

1st Sept 1992 to

30 April 1996

3 years  & 7 months

 

 

 

 

Conference Organized

Organizing Secretary

“New Frontiers in Drugs Discovery and Development against Emerging & Reemerging Viral Infections

National Symposium on Influenza: Epidemiology and Control,

April, 5th 2005. (On the eve of 56th foundation day of VPCI)

 

Award and Honours

  1. Bronze award (AVG Award) for the best paper in Ditan International Conference on Infectious Diseases, Beijing, China on July, 30-Aug 2, 2009.                                                     
  2. Young Scientist award, ESWI, 14-17 Sept, 2008, Portugal.
  3. Young investigator award in “Options for the Control of Influenza VI” Ontario, Canada 17-23 June, 2007(Oral presentation).

 

4.   BOYSCAST Fellowship from Department of Science and Technology, GOI in the 2000, to work at WHO Center for reference and research center Australia, Melbourne.

 

5.   DST Young Scientist independent scheme on Characterization of drug resistant clinical isolates of M.tuberculosis by PCR analysis. Cost Rs. 5,00 000

 

6.   CSIR Individual fellowship of Research Associate for the project entitled: Spontaneous Release of Interleukin-2 and Generation of Superoxide In Active Pulmonary Tuberculosis

 

7.   Shakuntala Amir Chand Award of Indian Council of Medical Research (I.C.M.R.) for the year 1992 for outstanding research work in the field of "Cytokine Mediated Pathology of Dengue-virus Infection".

 

8.   BEST POSTER AWARD during the XXII Annual Conference of Indian Immunology Society & National Symposium on Immunology of Infectious Disease, held during 15-17 Dec. 95, JNU, New Delhi, India.

 

 

 

Name of the Project

Funding  Agency

 

Date of Sanction

 

Total Approved

Cost of the Project

(in Rs.)

 

1.A combinatorial antiviral approach against influenza A virus using ribozymes and SiRNAs  ( PI)

 

Department of Biotechnology

 

Nov. 2006

 

   

40.22 Lakhs

 

 

2. A Study of Viral Replication

Inhibition by Down Regulation of NS1 Gene of    Influenza A Virus (PI)      

Council of Scientific and Industrial Research (CSIR), New Delhi

 

Oct 2007-Oct 2010

 

 

13.00 Lakhs

 

3. Multi-Site Monitoring of Human Influenza Viruses in India Phase-I”(PI)

 

Indian Council of Medical Research

Sep. 2006-28 Feb. 2010

 1 Crore

4. Typing and subtyping of influenza virus by RT-PCR (PI)

 

Department of Science & Technology

 

 

Dec.1998

 

 

 

3.75 Lakhs

 

 

5. Study of Virological and biochemical regulatory mechanism of influenza virus induced apoptosis in murine model of allergic asthma (PI)

Council of Scientific and Industrial Research (CSIR), New Delhi

Feb 2003

 

 

 

 

13.00 Lakhs

 

6. Genetic analysis of influenza virus by molecular techniques (PI)

 

Department of Science & Technology

 

Nov 2003

 

 

 

 

18.00 Lakhs.

 
         

 

 

Recently sanctioned Project

·       DST 45 Lakhs

                  Recombinant monoclonal antibodies against influenza A virus and pandemic     

                  influenza 2009.

 

·       DRDO 45.21 Lakhs

      Study of antigenic diversity and cross reactive antibody generation to influenza virus       

      In human samples.

 

 International Research Trainings

 

1.     3rd ICGEB workshop on Human RNA Viruses at Institute Leloir, Argentina from 2-5 April,2012.

 

2.     The Baltic Summer School, International Training Course on "Current Trends In Immunology And Signal Transduction" Including Practical Course On Immunology And Molecular Biology Techniques At University of Kiel, Germany from Sept.17-Oct.8, 2001.

 

3.     International Training Course on Cellular and Molecular Biology sponsored by International Research Organization at Wiezmann Institute, Israel on 10 Dec.1994 to 23 Dec.1994.  

 

4.     IUIS Advance Course on Basic Immunology held at All India Institute of Medical Sciences New Delhi on Nov. 22-27, 1993.

 

 

 

 

List of Publications

 

  1. Rajput R, Khanna M, Kumar P, Kumar B, Sharma S, Gupta N, Saxena L (2012) siRNA targeting the nonstructural gene (NS1) transcript inhibits influenza A virus replication in experimental mice. Nucleic Acid Ther. (DOI: 10.1089/nat.2012.0359)
  2. Kumar B, Kumar P, Rajput R, Daga MK, Singh V and Khanna M (2012). Comparative reproducibility of SYBR Green I and TaqMan real-time PCR chemistries for the analysis of matrix and hemagglutinin genes of Influenza A viruses. International Journal of Collaborative Research on Internal Medicine & Public Health 4(7): 1346-1352
  3. Kumar B, Pati DR, Khanna M, Kumar P, Daga MK, Singh V, Khare S, Gaur SN (2012). Age-sex distribution and seasonality pattern among influenza virus infected patients in Delhi, 2009-2010. Ind. J Com. Med., 37:57-58.
  4. Kumar P, Khanna M, Kumar B, Rajput R, Banerjea AC (2012).A conserved matrix epitope based DNA vaccine protects mice against influenza A virus challenge. Antiviral Res. 93:78-85.
  5. Srivastava V, Khanna M, Sharma S and Kumar B. Resolution of Immune Response by rTGF-β during influenza A virus infection. IJMR, 136: 641-648
  6. Kumar B, Khanna M, Kumar P, Sood V, Vyas R and Banerjea AC. Nucleic Acid Mediated Cleavage of M1 Gene of Influenza A Virus Is Significantly Augmented by Antisense Molecules Targeted to Hybridize Close to the Cleavage Site (2010). Mol. Biotechnol. 51:27-36.

7.     Kumar B, Khanna M, Kumar P, Gupta A, Daga MK, Sarkar MC, Chadha MS, Mishra AC and Kaur H (2011). Quantification of viral load in clinical specimens collected from different body sites of patients infected with influenza viruses. Int. J. Med. Med. Sci. 3 (5): 144-148

8.     Kumar P, Vyas R, Sood V, Gupta N, Banerjea A & Khanna M (2010). Potent    inhibition of influenza virus replication with novel siRNA-chimeric-ribozyme constructs. Antiviral Res. 87: 104-112.

  1. Kumar B, Sharma B, Khanna M, Singh V, Daga MK, Vijayan VK, Mishra AC,  Chadha MS, Sarkar MC & Kaur H (2010). Comparison of various immunoassay kits for rapid screening of pandemic influenza H1N1-2009 viruses. Journal of Public Health and Epidemiology 2:175-179.

10.  Kumar P., Kumar B., Gupta A., Sharma B., Vijayan V.K., Khare S., Singh V., Daga M.K., Chadha M.S., Mishra A.C., Kaur H., Khanna M (2010). Diagnosis of novel pandemic influenza virus 2009 H1N1 in hospitalized patients. Indian J Viro. 21:45-49.

11.  Khanna M, Ray A, Rawall S, Chandna S, Kumar B and Vijayan VK (2010). Detection of Influenza virus induced ultrasructural changes and DNA damage; I J Virol. 21:50-55.

12.  Khanna M, Rajput R, Kumar P, Sharma D, Mathur D, Prasad AK (2010). Potent inhibition of pandemic influenza H1N1 (2009) virus propagation by novel chemically synthesized compounds. Inf & Resp Viruses 5 (Suppl. 1), 90-123.

  1. Khanna M and Gupta A (2010). Vaccines for Pandemic Influenza A H1N1 (2009): Where Do We Stand? I J Virol.21:90-91.
  2. V. Srivastava, S. Rawall, V.K. Vijayan & M. Khanna (2009) Influenza A virus induced apoptosis: Inhibition of DNA laddering & caspase 3 activity by zinc supplementation in cultured HeLa cells Indian J Med Res 129: 115-122
  3. Khanna M, Kumar P K Chaudhry,B Kumar & V.K. Vijayan (2008) Emerging influenza virus: A Global Threat J Bio Sci; 33:475-482
  4. Khanna M, Gupta N,Gupta A & vijayan VK (2009).  Influenza 2009 H1N1 :apandemic alarm J Bio Sci 34(3), September 2009, 481–489,
  5. Nazir R, Khanna M & Kulshrestha R. Time Course of Pulmonary Pathology, Cytokine Influx and Their Correlation on Augmentation of Antigen Challenge by Influenza A Virus Infection. IJEB. 2008; 46: 151-158.
  6. Kumar P,  Srivastava V, Khanna M  and Vijayan V K, (2007)  Biphasic effect of immune r TGF-β1 on  inducible Nitric oxide Synthase during Influenza A virus infection in mice  Proceedings of Option for Control of Influenza 559-560
  7.  Khanna M, Kumar P, Vijayan V K, Singh V. (2007) Release of Macrophage inflammatory protein, IL-6 and Eosinophilic derived neurotoxin into upper respiratory secretions during influenza virus infection in children Ind J Chest Dis 49: 249-251.
  8. Kumar P, Khanna M, Tyagi Y.K, Srivastava V, Ravi K & Raj H.G. (2005) Effect of Quercetin supplementation on   antioxidant of lung after experimental influenza virus infection. Exp. Lung Res. 31 (5).
  9. Kumar P, Khanna M, Sharma S and Raj HG. (2003). Effect of Quercetin on    lipid peroxidation and changes in lung morphology in experimental influenza virus infection. Int. J. Exp.Path. 84; 1-7.
  10. Khanna M, Kumar P, Chhabra SK, Chugh P and Prasad AK (2001).  Evaluation of influenza virus detection by direct enzyme immunoassay (EIA) and conventional methods in asthmatic patients. J Commun Dis 33; 163-9.
  11. Khanna M, Akhter N, Srivastava V, Kumar P Vijayan V.K., (2006) Biological and Epidemiological Aspects of Influenza Virus H5N1 in Context of India. Ind. J. Exp. Biol., 44: 265-278.
  12. Khanna M, Kumar P, Srivastava LM. (2003) Defective Interelukin-2 and IL-2 receptor expression in pulmonary tuberculosis.  J Commun Dis. 35 (2) 65.
  13. Khanna M, Kumar P. (2002). Influenza: A serious global threat. J. Infect. Dis. Antimicro. Agents. 19; 25-39.
  14. Khanna M, Srivastava V and Kumar P. (2002). Virological studies in clinical practice: Utility & Indications. Medicine Update Annual Ed. 7/2; 74-78.
  15. Khanna M, Kumar P and Prasad AK. (2001). Influenza and its role in apoptosis IJAAI. 15; 7-12.

Chapters in international books

1.     Khanna M, Srivastava V., Structure and Classification of Influenza Virus. P 11 – 23. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6).

2.     Khanna M, Srivastava V., Clinical and Laboratory Diagnosis of Influenza Virus. P 138 – 151. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6).

3.     Khanna M, Nazir R, Srivastava V., Prophylaxis Prevention and Treatment for Influenza A,B,C. p 169 – 191. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6).

4.     Khanna M, Srivastava V., Influenza Pandemic Preparedness. P 192 – 201. In: Tripathi M. Mathew & Thankam Mathew, eds. Influenza and Its’ Global Public Health  Significance Thajema Publishers 31 Glen view Dr. West Orange NJ 07052 USA: (6)Post Doctoral Publication

1.     Khanna M, Kumar P, Srivastava LM. (2003) Defective Interlukin-2 and IL-2 receptor expression in pulmonary tuberculosis.  J Commun Dis.35 (2) 65-70.

2.   Sharma S.K., Khilnani,V. Pandey.V.C. and Khanna, M.(1995). Immunological and Pulmonary Function Abnormalties in tropical Pulmonary Eosinophilia. Indian Journal of Medical Research, 101:98-102.

3.   Sharma SK, Khanna M, Srivastava LM, Pandey JC and Khilnani V. (1995). Effect of corticosteriod treatment on various serological and bronchoalveolar   lavage (BAL) abnormalties in patients with sarcoidosis. IJMR. 101:207-212.

4.   Khanna M and Srivastava LM. (1996) Release of superoxide anion by mouse peritoneal macrophages during Mycobacterium tuberculosis infection. Ind. J. Exp. Biology.  34, 407-410.

5.   Khanna M, Goel A, Jain RC, and Srivastava LM. (1997). Rapid and simple method for detection of rifampicin resistant clinical isolates of M.  Tuberculosis. Ind. J. Clin. Biochemistry. 12 (suppl); 83-85.

 

 PhD Publication       

1.     Khanna M, Chaturvedi UC and Mathur A. (1988).  Abrogation of helper T cell by dengue virus induced cytotoxic factor. Current Science. 57; 411-414.

2.     Khanna M, Chaturvedi UC and Mathur A. (1989). Cytotoxic effects   of dengue virus induced cytotoxin on helper T cell. Ind. J. Med. Micro. 7; 54-57.

3.     Khanna M, Chaturvedi UC and Mathur A. (1989b). Proteinase like activity in the cytotoxic factor produced by T cells during dengue virus infection. Immunology. 62; 32-37.

4.     Dhawan R, Khanna M, Chaturvedi UC and Mathur A. (1990). Effects of dengue virus-induced cytoxin on capillary permeability. Br. J.Exp.Path. 71; 83-89.

5.     Khanna M, Chaturvedi UC and Mathur A. (1990). Increased capillary permeability mediated by dengue virus-induced cytokine. Immunology. 69; 449-453.

6.     Dhawan R, Khanna M, Chaturvedi UC and Mathur A. (1990). Role of calcium in induction and secretion dengue virus-induced cytokines. J. Bio. Sciences. 15; 409-416.

7.     Dhawan R, Chaturvedi UC, Khanna M, Mathur A, Tekwani BL, Pandey VC and Rai RN. (1991).  Obligatory role of calcium in the cytotoxic activity of dengue virus-induced cytotoxin.  Br.  J.  Exp.   Pathol. 72; 31-39.

8.     Khanna M, Chaturvedi UC, Dhawan R, Tekwani BL and Pandey VC. (1991). Presence of calcium is obligatory for the cytotoxic activity of dengue virus-induced cytotoxic factor.  Immunology. 72; 73-78. 

9.     Chaturvedi UC, Dhawan R and Khanna M. (1991). Breakdown of blood brain barrier by dengue virus-induced cytotoxic factor. J Gen. Virology. 72; 859-866.

10.  Khanna M and Chaturvedi UC. (1992). Purification of amino acid terminal sequence of the dengue virus-induced cytotoxic factor. Br. J Expt. Pathol. 73; 43-49.

11.  Dhawan R, Chaturvedi UC and Khanna M.  (1994). Breakage of blood brain barrier    by macrophage cytotoxin. Proc. Indian Natl. Sci. Acad. 60; 45-52.     

Total Publications

International Journals: 22

National Journals: 22

    Book Chapters:  4